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a 431  (ATCC)


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    ATCC a 431
    A 431, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 3750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+431/A-431/pm42235302-221-1-19
    Average 98 stars, based on 3750 article reviews
    a 431 - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    In Vitro:

    Article Title: Evaluation of the Functional Suitability of Carboxylate Chlorin e 6 Derivatives for Use in Radionuclide Diagnostics
    Article Snippet: .. The in vitro tumor cell accumulation of the 99m Tc-labeled chlorins was assessed using SKOV-3 (ovarian adenocarcinoma) and A-431 (epidermoid carcinoma) cell lines (ATCC). .. The cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Biowest, Nuaille, France).

    Article Title: Evaluation of the Functional Suitability of Carboxylate Chlorin e6 Derivatives for Use in Radionuclide Diagnostics
    Article Snippet: .. The in vitro tumor cell accumulation of the 99mTc-labeled chlorins was assessed using SKOV-3 (ovarian adenocarcinoma) and A-431 (epidermoid carcinoma) cell lines (ATCC). .. The cells were cultured in Roswell Park Memorial Institute (RPMI)-1640 medium (Biowest, Nuaille, France).

    Cell Culture:

    Article Title: AAK1-mediated phosphorylation of PDLIM5 and Talin1 promotes focal adhesion disassembly to accelerate cell migration.
    Article Snippet: .. Cell lines and cell culture All cell lines were obtained from ATCC: hTERT-RPE1 (ATCC CRL-4000)(70), MDA-MB-231 (ATCC HTB-26), SH-SY5Y (ATCC CRL-2266), A-431 (ATCC CRL-1555), Saos-2 (ATCC HTB-85) and cultured in DMEM, high glucose, Glutamax medium (31966-021, Thermo Fisher Scientific-Gibco) supplemented with 10% FBS (Sigma-Aldrich, F7524). .. Cell lines and cell culture All cell lines were obtained from ATCC: hTERT-RPE1 (ATCC CRL-4000)(70), MDA-MB-231 (ATCC HTB-26), SH-SY5Y (ATCC CRL-2266), A-431 (ATCC CRL-1555), Saos-2 (ATCC HTB-85) and cultured in DMEM, high glucose, Glutamax medium (31966-021, Thermo Fisher Scientific-Gibco) supplemented with 10% FBS (Sigma-Aldrich, F7524).

    Multiple Displacement Amplification:

    Article Title: AAK1-mediated phosphorylation of PDLIM5 and Talin1 promotes focal adhesion disassembly to accelerate cell migration.
    Article Snippet: .. Cell lines and cell culture All cell lines were obtained from ATCC: hTERT-RPE1 (ATCC CRL-4000)(70), MDA-MB-231 (ATCC HTB-26), SH-SY5Y (ATCC CRL-2266), A-431 (ATCC CRL-1555), Saos-2 (ATCC HTB-85) and cultured in DMEM, high glucose, Glutamax medium (31966-021, Thermo Fisher Scientific-Gibco) supplemented with 10% FBS (Sigma-Aldrich, F7524). .. Cell lines and cell culture All cell lines were obtained from ATCC: hTERT-RPE1 (ATCC CRL-4000)(70), MDA-MB-231 (ATCC HTB-26), SH-SY5Y (ATCC CRL-2266), A-431 (ATCC CRL-1555), Saos-2 (ATCC HTB-85) and cultured in DMEM, high glucose, Glutamax medium (31966-021, Thermo Fisher Scientific-Gibco) supplemented with 10% FBS (Sigma-Aldrich, F7524).

    other:

    Article Title: Interactions of hop xanthohumols with model membranes and their toxicity to cancer and erythrocytes cells.
    Article Snippet: Prenylated hydroxychalcones (xanthohumols) are hop-derived flavonoids with promising anticancer activity; however, their membrane interactions and structure–activity relationships remain incompletely understood.. Here, xanthohumol C (XHC) and its semi-synthetic derivatives, 1′′,2′′-dihydroxanthohumol C (DHXHC) and 1′′,2′′dihydroxanthohumol K (DHXHK), were evaluated for cytotoxic, pro-apoptotic, and membrane-modulating effects in comparison with xanthohumol (XH).. In vitro antiproliferative activity against eleven human and one murine cancer cell lines yielded IC50 values in the micromolar range, with XHC showing the highest activity toward epidermoid carcinoma, urinary bladder carcinoma, and glioblastoma cells.



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    In vitro cytotoxicity and tumor-restricted replication analysis of KLS-3021 in cSCC cells (A) Schematic representation of KLS-3021 showing deletions of C11R , K3L , and J2R loci and insertions of therapeutic genes encoding PH-20, IL-12, and sPD1-Fc. (B) Cytotoxicity of KLS-3021 in human cSCC cell lines <t>(A-431</t> and HSC-1) and normal human epidermal keratinocytes (NHEKs) was measured via CCK-8 assay at 72 h post-infection. (C) Viral replication in cSCC cell lines and NHEKs was determined via TCID 50 assay at 72 h post-infection. Data was represented as mean ± SEM of three independent experiments.
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    Image Search Results


    In vitro cytotoxicity and tumor-restricted replication analysis of KLS-3021 in cSCC cells (A) Schematic representation of KLS-3021 showing deletions of C11R , K3L , and J2R loci and insertions of therapeutic genes encoding PH-20, IL-12, and sPD1-Fc. (B) Cytotoxicity of KLS-3021 in human cSCC cell lines (A-431 and HSC-1) and normal human epidermal keratinocytes (NHEKs) was measured via CCK-8 assay at 72 h post-infection. (C) Viral replication in cSCC cell lines and NHEKs was determined via TCID 50 assay at 72 h post-infection. Data was represented as mean ± SEM of three independent experiments.

    Journal: Molecular Therapy Oncology

    Article Title: KLS-3021: Innovative oncolytic virotherapy for the treatment of advanced primary and metastatic cutaneous squamous cell carcinoma

    doi: 10.1016/j.omton.2026.201182

    Figure Lengend Snippet: In vitro cytotoxicity and tumor-restricted replication analysis of KLS-3021 in cSCC cells (A) Schematic representation of KLS-3021 showing deletions of C11R , K3L , and J2R loci and insertions of therapeutic genes encoding PH-20, IL-12, and sPD1-Fc. (B) Cytotoxicity of KLS-3021 in human cSCC cell lines (A-431 and HSC-1) and normal human epidermal keratinocytes (NHEKs) was measured via CCK-8 assay at 72 h post-infection. (C) Viral replication in cSCC cell lines and NHEKs was determined via TCID 50 assay at 72 h post-infection. Data was represented as mean ± SEM of three independent experiments.

    Article Snippet: Luciferase-labeled A-431 cells were cultured in DMEM (ATCC) supplemented with 10% FBS (Gibco), 1% penicillin-streptomycin (Sigma), and 8 μg/mL blasticidin (InvivoGen).

    Techniques: In Vitro, CCK-8 Assay, Infection

    Tumor growth analysis in orthotopic primary cSCC model following KLS-3021 treatment (A) Schematic of the experimental design for the orthotopic cSCC model established by intradermal implantation of A-431 cells. (B) Tumor growth curves of vehicle-treated and KLS-3021-treated mice (mean ± SEM, n = 6 per group; ∗∗∗ p < 0.001 by two-tailed t test). (C) Individual tumor growth curves for each mouse. (D) Body-weight changes during the observation period were represented (mean ± SEM). (E and F) Representative hematoxylin and eosin (H&E)-stained sections of tumors collected on days 3 (E) and 7 (F) post-treatment. Scale bars, 300 μm; enlarged views, 100 μm.

    Journal: Molecular Therapy Oncology

    Article Title: KLS-3021: Innovative oncolytic virotherapy for the treatment of advanced primary and metastatic cutaneous squamous cell carcinoma

    doi: 10.1016/j.omton.2026.201182

    Figure Lengend Snippet: Tumor growth analysis in orthotopic primary cSCC model following KLS-3021 treatment (A) Schematic of the experimental design for the orthotopic cSCC model established by intradermal implantation of A-431 cells. (B) Tumor growth curves of vehicle-treated and KLS-3021-treated mice (mean ± SEM, n = 6 per group; ∗∗∗ p < 0.001 by two-tailed t test). (C) Individual tumor growth curves for each mouse. (D) Body-weight changes during the observation period were represented (mean ± SEM). (E and F) Representative hematoxylin and eosin (H&E)-stained sections of tumors collected on days 3 (E) and 7 (F) post-treatment. Scale bars, 300 μm; enlarged views, 100 μm.

    Article Snippet: Luciferase-labeled A-431 cells were cultured in DMEM (ATCC) supplemented with 10% FBS (Gibco), 1% penicillin-streptomycin (Sigma), and 8 μg/mL blasticidin (InvivoGen).

    Techniques: Two Tailed Test, Staining

    Bioluminescence imaging and tissue analysis in metastatic cSCC model following KLS-3021 treatment (A) Schematic of the experimental design for the orthotopic metastatic cSCC model established by intradermal implantation of luciferase-labeled A-431 cells into the footpad. (B) Longitudinal quantification of total photon flux from the primary tumor and popliteal lymph node (PLN) at each time point post-treatment ( n = 5 per group; ∗∗∗ p < 0.001 by two-tailed t test). (C) Representative IVIS bioluminescence images showing signals from primary and nodal tumors in vehicle- and KLS-3021-treated mice at indicated time points. (D and E) Primary tumor weights (D) and PLN weights (E) measured on day 3 ( n = 5 per group; ∗ p < 0.05 by two-tailed t test) and day 28 ( n = 10 per group; ∗ p < 0.05, ∗∗∗ p < 0.001 by two-tailed t test) post-treatment. All data were represented as mean ± SEM.

    Journal: Molecular Therapy Oncology

    Article Title: KLS-3021: Innovative oncolytic virotherapy for the treatment of advanced primary and metastatic cutaneous squamous cell carcinoma

    doi: 10.1016/j.omton.2026.201182

    Figure Lengend Snippet: Bioluminescence imaging and tissue analysis in metastatic cSCC model following KLS-3021 treatment (A) Schematic of the experimental design for the orthotopic metastatic cSCC model established by intradermal implantation of luciferase-labeled A-431 cells into the footpad. (B) Longitudinal quantification of total photon flux from the primary tumor and popliteal lymph node (PLN) at each time point post-treatment ( n = 5 per group; ∗∗∗ p < 0.001 by two-tailed t test). (C) Representative IVIS bioluminescence images showing signals from primary and nodal tumors in vehicle- and KLS-3021-treated mice at indicated time points. (D and E) Primary tumor weights (D) and PLN weights (E) measured on day 3 ( n = 5 per group; ∗ p < 0.05 by two-tailed t test) and day 28 ( n = 10 per group; ∗ p < 0.05, ∗∗∗ p < 0.001 by two-tailed t test) post-treatment. All data were represented as mean ± SEM.

    Article Snippet: Luciferase-labeled A-431 cells were cultured in DMEM (ATCC) supplemented with 10% FBS (Gibco), 1% penicillin-streptomycin (Sigma), and 8 μg/mL blasticidin (InvivoGen).

    Techniques: Imaging, Luciferase, Labeling, Two Tailed Test